Table 2.

Two way ANOVA analyses of all studied parameters in all groups regarding gender, Zn treatment, and gender versus ZnSO₄ treatment.

Studied parameters	Zn-treatment		Gender		Zn-treatment/gender
	F	p	F	P	F	p
Total weight	118.65	˂ 0.0001	746.18	˂ 0.0001	5.41	0.0002
Serum markers
Zn	53.72	˂ 0.0001	380.63	˂ 0.0001	11.51	˂ 0.0001
Leptin	228.51	˂ 0.0001	2045.89	˂ 0.0001	45.45	˂ 0.0001
Glucose level	80.56	˂ 0.0001	0.00	0.9756	3.74	0.0034
Brain markers
Leptin receptor	228.51	˂ 0.0001	2045.89	˂ 0.0001	45.45	˂ 0.0001
PDNF	1246.42	˂ 0.0001	465.32	˂ 0.0001	14.46	˂ 0.0001
p-STAT3	283.27	˂ 0.0001	155.63	˂ 0.0001	4.87	0.0004
AChE	6.63	˂ 0.0001	61.75	˂ 0.0001	33.81	˂ 0.0001
Lipid profile
Total Lipids	23.4	˂ 0.0001	365.91	˂ 0.0001	2.91	0.0158
Cholesterol	20.18	˂ 0.0001	147.03	˂ 0.0001	1.21	0.3072
Triglycerides	110.47	˂ 0.0001	128.92	˂ 0.0001	15.24	˂ 0.0001
Liver enzymes
SGOT	34.78	˂ 0.0001	150.27	˂ 0.0001	15.22	˂ 0.0001
SGPT	134.15	˂ 0.0001	445.15	˂ 0.0001	37.49	˂ 0.0001
Kidney functions test
Urea	16.3	˂ 0.0001	4.54	0.0350	0.51	0.7681
Uric acid	39.25	˂ 0.0001	9.38	0.0027	11.90	˂ 0.0001
Creatinine	2.02	0.0798	7.83	0.0059	1.55	0.1791