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. 2023 Mar 15;14:1141712. doi: 10.3389/fimmu.2023.1141712

Figure 3.

Figure 3

Viability & frequencies of MHC-II+, CD86+ & CD40+ nDCs and iDCs exposed to 10 µg/ml αCD40 and 1µM ISO for 48h. (A) A significant decrease in the viability of αCD40 treated nDCs with ISO treatment was observed in absence of tumor lysate stimulation. (B, C). The population of MHC-II+ and CD86+ nDC and iDC was reduced with ISO and αCD40 co-treatment, however, CD40+ population remain unchanged in nDCs but reduced in iDCs. (D) Decrease in the surface expression of co-stimulatory molecules was significantly higher in αCD40-treated iDCs compared to nDCs. Fold change in a cell population with ISO treatment was calculated using αCD40 only treated nDCs and iDCs as control and using the formula: [(ISO treated population/Control population)-1]. (E) The results are demonstrated by representative contour plots of MHC-II+, CD86+ and CD40+ cell populations, showing the intensity of ISO treated nDCs and iDCs overlaid with their respective αCD40 treatment controls. Statistical analysis was carried out using unpaired T-test, One-way ANOVA & Two-way ANOVA tests where applicable. P values less than 0.05 were considered significant. *** P <0.0005, **** P <0.0001.