Table 1.

List of assigned peaks of refucosylated Remab6 N-glycans from MALDI-TOF–MS analysis.

Composition	m/z	Relative intensity %
		0.1 μM	0.5 μM	1 μM	5 μM	10 μM	50 μM
Hex3 HexNAc3	1417	3.54	4.20	4.38	3.00	0.46	0.52
Hex5 HexNAc2	1580	7.52	2.20	5.06	3.61	1.45	2.80
Fuc1 Hex3 HexNAc3	1591	0.31	0.52	0.59	1.45	1.41	1.55
Hex3 HexNAc4	1662	66.18	77.08	68.65	44.94	5.74	0.33
Fuc1 Hex3 HexNAc4	1836	0.69	3.37	5.31	35.22	72.59	68.49
Hex4 HexNAc4	1866	19.21	11.23	13.72	5.92	2.34	0.42
Fuc1 Hex4 HexNAc4	2040	0.97	0.84	1.51	5.27	14.87	24.16
Hex5 HexNAc4	2070	1.38	0.42	0.58	0.31	0.23	0.10
Fuc1 Hex5 HexNAc4	2244	0.20	0.14	0.21	0.29	0.90	1.63
% Refucosylation		2.17	4.87	7.62	42.22	89.77	95.83

Amount of L-fucose indicated in μM. Fucosylated glycans noted in bold. Raw mass spectrometry data provided in Supplementary Table S1.