Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 27;128(7):1236–1248. doi: 10.1038/s41416-023-02154-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Immunocytochemical (ICC) images of Hep3B-DOX-L1 ORF1 cells costained with PIN1-AF-549 and L1 ORF1 + AF488 48 h after Dox induction. Nuclei were counterstained with DAPI. Untreated cells were used as controls. Co-localisation analysis for Pin1 and L1 ORF1p revealed Manders coefficient M1 (proportion of the intensity from the green channel that co-localises with the red channel) = 0.958 and M2 (proportion of the intensity from the red channel that co-localises with the green channel) = 0.97; Pearson’s coefficient (co-variance of the two channels) = 0.898 for the image. b ICC images showing pSMAD3 staining of Hep3B-DOX-Empty and Hep3B-DOX-L1 ORF1 cells 48 h after Dox induction. Cells were stained with mouse-anti-pSMAD3 antibody followed by anti-mouse-AF647 secondary antibody. DAPI was used to counterstain the nuclei. MFI = mean fluorescent intensity of the indicated channel (n = 20). ****P < 0.0001 for AF647 channel, unpaired t test. c Enrichment plots obtained by GSEA upon analysing RNAseq data comparing Hep3B-DOX-L1 ORF1 versus Hep3B-DOX-Empty cells. The significance cut-off was set at FDR < 25% and p < 0.05. d Interaction map of PIN1 with differentially expressed genes in Hep3B-DOX-L1 ORF1 versus Hep3B-DOX-Empty cells. Shades of green represent downregulated and red represents upregulated genes. The orange colour of PIN1 indicates IPA predicts activation of PIN1 activity. e Graphs representing RT-qPCR results of indicated transcripts in Hep3B-DOX-Empty and Hep3B-DOX-L1 ORF1 cells upon Dox induction (1 µg/ml for 48 h) alone or in combination with the indicated inhibitor for the last 24 h. 18S was used as a normalisation control. Values represent mean ± SE of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-sample t test with a theoretical mean of 1.