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. 2023 Mar 15;15:1120952. doi: 10.3389/fnagi.2023.1120952

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Copyright © 2023 Birkle and Brown.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LPS-induced neuronal loss is dependent on microglia. (A) Average microglial counts per image in neuron-glia cultures treated ± BAY61 (1 μM) and ± LPS (100 ng/ml) for 3 days (DIV10), after previous ± LME treatment to deplete microglia. (B) Average DIV10 neuronal counts per image in neuron-glia cultures treated ± BAY61 (1 μM) and ± LPS (100 ng/ml) for 3 days (DIV10), after previous ± LME treatment to deplete microglia. All panels: each datapoint represents the mean of 3 technical replicates. Number of biological repeats = number of datapoints per column. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. RM 2-way ANOVA with Šídák’s post-hoc test.