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. 2023 Mar 15;14:1130308. doi: 10.3389/fimmu.2023.1130308

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Copyright © 2023 Zhuang, Cao, Liu, Wang, Lin, Duan, Lee, Chiu, Yeung and Yao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of EVT from JEG-3-ORGs with the effect of HLA-G. (A) Quantification of the relative mRNA expression of HLA-G, MMP2, ITGA5, CDH3, and IGF2 at the EVT differentiation stage of two kinds of JEG-3-ORGs (KO-ORGs and WT-ORGs). (B) Temporal expression of the relative mRNA quantification for the acknowledged EVT-classified genes after exogenous addition of HLA-G protein-based KO-ORGs at different concentrations (0.025 µg/ml, 0.05 µg/ml, and 0.1 µg/ml). ***P<0.001; ns, not significant. n = 3 in triplicate.