Table 2.
Primers for RT-qPCR analysis.
| Gene1 | Primer sequence (5′–3′)2 | Length (nt) | GenBank number |
|---|---|---|---|
| OCLN | F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGCAGATGAG |
123 | XM_025144247.2 |
| CLDN2 | F: CCTACATTGGTTCAAGCATCGTGA R: GATGTCGGGAGGCAGGTTGA |
131 | NM_001277622.1 |
| TNF-α | F: GAGCGTTGACTTGGCTGTC R: AAGCAACAACCAGCTA TGCAC |
176 | NM_214022.1 |
| IL-1β | F: ACTGGGCA TCAAGGGCTA R: GGTAGAAGA TGAAGCGGGTC |
154 | NM_214005.1 |
| IL-10 | F: AGAAATCCCTCCTCGCCAAT R: AAATAGCGAACGGCCCTCA |
121 | NM_001004414.2 |
| IL-6 | F: GCTGCGCTTCTACACAGA R: TCCCGTTCTCA TCCA TCTTCTC |
203 | NM_204628.1 |
| GAPDH | F: TGCTGCCCAGAACATCATCC R: ACGGCAGGTCAGGTCAACAA |
142 | NM_204305 |
1
OCLN = occludin; CLDN2 = claudin2; TNF-α = tumor necrosis factor-α; IL-1β = interleukin-1β; IL-10 = interleukin-10; IL-6 = interleukin-6; GAPDH = glyceraldehyde-3-phosphate.
2
F = forward primer; R = reverse primer.