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. 2023 Mar 15;13:1125901. doi: 10.3389/fcimb.2023.1125901

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Copyright © 2023 Rowe, Lebman and Ohman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of added alginate on the phagocytosis of nonmucoid P. aeruginosa. (A) MH-S macrophages were infected with GFP-expressing FRD1 or FRD1131 for 30 min in the presence of increasing concentrations of bacterial alginate. Percent phagocytosis indicates the percent of macrophages that are associated with bacteria as detected by flow cytometry. Phagocytosis of FRD1131 (Alg^-) was not significantly inhibited by the addition of FRD1 alginate. Data are shown as mean and standard deviation of 3 separate experiments. (B) MH-S macrophages were infected with FRD1, FRD1131 or both strains combined for 1 h. CFU indicates the number of colony-forming units recovered from macrophages. Phagocytosis of FRD1131 was not significantly inhibited by the addition of FRD1. Data are shown as mean and standard deviation of three separate experiments.