Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 15;13:1125901. doi: 10.3389/fcimb.2023.1125901

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Rowe, Lebman and Ohman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of alginate production on IL-8 and MIP-2 production. (A) To determine the effect of alginate on IL-8 production, THP-1 macrophages were infected with FRD1 or FRD1131, or treated with FRD1 or FRD1131 supernatants (Supe) for 8 h. Supernatants were collected and analyzed by ELISA for IL-8 production. No significant difference was found between the amount of IL-8 induced by FRD1 compared to FRD1131, nor their supernatants. Data are shown as mean and standard deviation of 3 separate experiments. (B) To determine the effect of alginate on MIP-2 production, MH-S macrophages were infected with FRD1, FRD1131, or treated with FRD1 or FRD1131 supernatants for 8 h. Supernatants were collected and analyzed by ELISA for MIP-2 production. No significant difference was found in the amount of IL-8 induced by FRD1 compared to FRD1131, nor by their respective supernatants. Data are shown as mean and standard deviation of three separate experiments. (C) To examine contact dependence on MIP-2 production, MH-S macrophages were infected with FRD1 or FRD1131 either directly or above a filter for 8 h. Supernatants were collected and analyzed by ELISA for MIP-2 production. No significant difference was found between the amount of IL-8 induced by FRD1 compared to FRD1131, nor when separated by a filter (although the filters changed the volume which lowered the overall MIP-2 concentration). Data are shown as mean and ANOVA analysis of three separate experiments.