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. 2023 Mar 15;14:1160162. doi: 10.3389/fpls.2023.1160162

Figure 2.

Figure 2

At the subcellular level, mCherry-FYVE3 is mainly cytosolic but can be found at the PI3P-enriched membrane of autophagic and endosomal organelles. (A-F), Representative images of root tip cells co-expressing mCherry-FYVE3 with a variety of organelle markers, such as PI3P biosensor (A), late endosome marker (B), phagophore and autophagosome marker (C), trans-Golgi network marker (D), Golgi stack marker (E), and GFP-FYVE2 (F), which associates with autophagic and endosomal membranes. Scale bars = 5 µm. Arrows indicate mCherry-FYVE3 puncta that overlap with co-expressed markers, whose quantification is shown in (G). Columns marked with different letters represent significantly different means according to the t-test (mean ± S.E.; n = 11–12 images; p < 0.01).