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. 2023 Mar 15;13:1123650. doi: 10.3389/fcimb.2023.1123650

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Copyright © 2023 Wang, Zeng, Yin, Yin, Shen, Xu, Dai and Pan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of wogonin in suppressing PEDV replication in Vero cells. (A, B) Inhibitory effects of wogonin (12.5–100 μM) on PEDV-infected cells (MOI = 0.01) observed by IFA (A). Scale bar, 200 μm. Cell lysate was harvested for TCID50 assay (B). (C, D) Vero and IPEC-J2 cell culture supernatant were collected for RT-qPCR after incubation with serially diluted wogonin for 48 h. (E, F) The IC50 values were calculated using GraphPad Prism software 7.0 based on the RT-qPCR results. Bars represent the SD of triplicate trials (* denotes significant difference between test concentration and control; ns, no significant; *P < 0.05; **P < 0.01; ***P < 0.001).