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. 2023 Mar 15;13:1123650. doi: 10.3389/fcimb.2023.1123650

Figure 5.

Figure 5

Wogonin blocks Mpro to disrupt PEDV infection. (A) Stability of Mpro determined by DSF assay. For the DSF assay, 1 mg/mL Mpro and 40 mM wogonin were employed. (B, C) Binding of Mpro to wogonin was investigated by MST assay. For MST assay, 40µM–1.22 nM wogonin and 200 nM Mpro were employed. (D) Binding curve of wogonin with Mpro in surface plasmon resonance (SPR) assay.