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. 2023 Mar 15;13:1123650. doi: 10.3389/fcimb.2023.1123650

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Copyright © 2023 Wang, Zeng, Yin, Yin, Shen, Xu, Dai and Pan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of the PEDV Mpro enzymatic activity by wogonin. (A) The fluorescence resonance energy transfer (FRET) assay was performed to analyze the enzymatic activity of the purified PEDV Mpro. (B) The effect of wogonin on the activity of Mpro by FRET assay. IC50 was determined using GraphPad Prism 6 software. Data are shown as the means ± SD of three independent experiments.