Figure 1.  . Glutaminase family of enzymes share high degree of homology in sequence and catalytic mechanism.

(A) Glutamine hydrolysis as catalyzed by glutaminase enzymes. (B) Glutaminase enzymes are encoded by two independent genes, GLS and GLS2. (C) Mechanism of the hydrolytic reaction of the substrate, glutamine, as catalyzed by the active site residues in glutaminase enzymes. All three active forms of glutaminase share the same active site residues, which are numbered according to human GAC (and human LGA).

C-ter: C-terminus; MLS: Mitochondrial localization sequence.