Table 1.

Primer pairs and PCR conditions used for the amplification of the genes included in this study.

Gene 1	Primer Pairs 2	PCR Cycling Program (T-Time)
		Initial Denaturation	Amplification				Final Extension
			N° of Cycles	Denaturation	Annealing	Extension
Fungal isolates
ITS	ITS4/ITS5	94 °C-3 min	35	94 °C-30 s	48 °C-30 s	72 °C-45 s	72 °C-10 min
TUB	Bt2a/Bt2b	95 °C-3 min	35	94 °C-15 s	55 °C-15 s	72 °C-1 min	72 °C-7 min
LSU	LR0R/LR7	95 °C-3 min	35	94 °C-15 s	52 °C-15 s	72 °C-1 min	72 °C-7 min
Bacterial isolates
16S rDNA	27F/1492R	94 °C-3 min	30	94 °C-30 s	55 °C-30 s	72 °C-1 min	72 °C-10 min

^1,2 ITS = internal transcribed spacer 1–5.8S rDNA-internal transcribed spacer 2 [30]; TUB = partial β-tubuline gen [31]; LSU = partial 28S rDNA gene [32,33]; 16S rDNA gene [34].