Table 2.
Species | Isolate 1 | Host (Plant) | Substrate/Conditions 2 | Genbank Accession 3 |
Blast Accession 4 |
Query Length | Gaps 5 | Identities 6 | Maximum Identity (%) |
---|---|---|---|---|---|---|---|---|---|
Internal Transcribed Spacer Region Gene (Fungal and Yeast Isolates) | |||||||||
Aspergillus flavus | MZC-17 | Eucalyptus sp. | Wood/Field colonization | MN069570 | KX462773 | 589 | 1/584 | 58/3/584 | 99.8 |
MZC-20 | Pinus pinea | Wood/Laboratory colonization | MN069573 | MH485381 | 586 | 0/581 | 581/581 | 100 | |
Aspergillus niger | MZC-14 | Pinus pinea | Wood/Laboratory colonization | MN069568 | KM259827 | 626 | 1/599 | 599/599 | 100 |
MZC-18 | Pinus pinea | Wood/Laboratory colonization | MN069571 | KM259827 | 623 | 0/623 | 623/623 | 100 | |
Chaetomium globosum | MZC-2 | - | Filter paper/Laboratory colonization | MN069561 | KR014363 | 603 | 1/496 | 493/493 | 100 |
MZC-9 | - | Filter paper/Laboratory colonization | MN069565 | KM268665 | 606 | 0/602 | 602/602 | 100 | |
Doratomyces stemonitis | MZC-16 | Populus alba | Wood/Laboratory colonization | MN069569 | FR799473 | 588 | 2/588 | 583/588 | 99.1 |
Mucor circinelloides | MZC-5 | - | Suspension from collected termites | MN069563 | HM641688 | 668 | 1/657 | 650/657 | 99.0 |
MZC-23 | Populus alba | Wood/Laboratory colonization | MN069575 | JN561250 | 627 | 1/627 | 624/627 | 99.5 | |
Mucor fragilis | MZC-1 | - | Suspension from collected termites | MN069560 | GU566275 | 664 | 1/662 | 659/662 | 99.5 |
Penicillium chrysogenum | MZC-0 7 | - | - | MN069559 | KC009774 | 612 | 2/612 | 610/612 | 99.7 |
Penicillium citrinum | MZC-21 | Pinus pinea | Wood/Laboratory colonization | MN069574 | KC344966 | 538 | 0/538 | 538/538 | 100 |
Pichia guilliermondii | MZC-19 (Yeast) | Pinus pinea | Wood/Laboratory colonization | MN069572 | EF192233 | 631 | 4/621 | 616/621 | 99.1 |
Trichoderma asperellum | MZC-8 | Eucalyptus sp. | Wood/Field colonization | MN069564 | KM268676 | 622 | 0/615 | 615/615 | 100 |
MZC-10 | Eucalyptus sp. | Wood/Field colonization | MN069566 | EU280110 | 631 | 1/629 | 627/629 | 100 | |
MZC-11 | Eucalyptus sp. | Wood/Field colonization | MN069567 | MH351209 | 591 | 0/589 | 588/589 | 99.8 | |
MZC-24 | Eucalyptus sp. | Wood/Field colonization | MN069576 | EU280110 | 631 | 2/621 | 618/621 | 99.5 | |
Trichoderma longibrachiatum | MZC-4 | Eucalyptus sp. | Wood/Field colonization | MN069562 | KJ174230 | 622 | 2/623 | 621/623 | 99.7 |
16S rDNA gene (Bacteria isolates) | |||||||||
Burkholderia kirstenboschensis | MZC-3 | Populus alba | Wood/Laboratory colonization | NM069326 | HF674708 | 1367 | 2/1366 | 1353/1366 | 99.0 |
Klebsiella aerogenes | MZC-13 | - | Suspension from collected termites | NM069330 | MH368390 | 1391 | 1/1391 | 1389/1391 | 99.9 |
Lactococcus lactis subsp. cremoris | MZC-7 | - | Filter paper/Laboratory colonization | NM069328 | MK290341 | 1006 | 0/1006 | 1005/1006 | 99.9 |
Serratia marcescens | MZC-6 | Pinus pinea | Wood/Laboratory colonization | NM069327 | CP018929 | 1397 | 0/1397 | 1397/1397 | 100 |
MZC-12 | Pinus pinea | Wood/Laboratory colonization | NM069329 | MK157269 | 1012 | 1/1012 | 1008/1012 | 99.6 | |
MZC-22 | Pinus pinea | Wood/Laboratory colonization | NM069331 | KR817904 | 1403 | 1/1403 | 1401/1403 | 99.9 |
1 MZC: Collection of Microbiology and Zoology Departments of UCO of cellulolytic microorganisms. 7 Positive control in cellulolytic activity test. 2 Substrates and conditions from which microorganisms associated with Reticulitermes grassei were isolated: from decomposed wood of forest trees colonized by R. grassei (Wood/Field colonization); from food sources after being eaten by R. grassei in laboratory-controlled conditions (Filter paper or Wood/Laboratory colonization), or from a suspension of collected termites (R. grassei) from affected fields (Suspension from collected termites). 3 Corresponding GenBank accession numbers of our isolates. 4 GenBank accession numbers blasted with the isolates obtained in this study. 5 Number of spaces introduced into the alignment to compensate for insertions and deletions in our sequence relative to blasted sequences. 6 Number of nucleotides of our sequences/number of nucleotides of blasted sequences.