Figure 6.

Activation of IFN-γ responses following vaccination and PD-L1 blockade. (A,B) IFN-γ responses to BRSV (A) or BVDV-1 antigen (B) before vaccination (on day 14) or after boost vaccination (at day 70). PBMCs isolated from calves that had been administered Calfwin 6 only (left, n = 6) or Calfwin 6 and anti-PD-L1 Ab (right, n = 6) on days 14 and 70 were cultured in the presence of BRSV or BVDV-1 antigen for seven days. IFN-γ production from PBMCs isolated from each animal was measured by ELISA in duplicate. Significant differences between groups were determined using the Wilcoxon signed-rank sum test.