Figure 4.

Titration of SARS-CoV-2 targeting crRNAs. The efficiency of 14 crRNAs was tested in HEK293T cells by co-transfecting them with the luciferase construct encoding SARS-CoV-2 +RNA target sequences. A titration of the crRNA constructs was performed (75, 150 and 300 ng). To quantify viral gene expression, luciferase level was measured at two days after transfection. Mean values (±SD) of three experiments in duplicate are shown. The average luciferase levels are expressed as percentage (%) of luciferase expression, setting the negative control (NC) at 100%. Statistical significance was determined using one-way ANOVA, * p ≤ 0.05.