Figure 5.

The anti-SARS-CoV-2 efficiency of single versus dual crRNA therapy. The efficiency of six crRNAs was tested in HEK293T cells by co-transfecting them with the luciferase construct encoding SARS-CoV-2 +RNA target sequences. The crRNAs were tested as single inhibitor versus a dual-combinatorial approach. To quantify viral gene expression, luciferase level was measured at two days after transfection. Mean values (±SD) of three experiments in duplicates are shown. The average luciferase levels are expressed as percentage (%) of luciferase expression, setting the negative control (NC) at 100%. Statistical significance was determined using one-way ANOVA, **** p ≤ 0.0001.