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. 2023 Mar 12;13(3):764. doi: 10.3390/life13030764

Figure 2.

Figure 2

Construction of chitinase gene silencing strain in C. militaris. (A) Construction of chitinase silencing plasmid. The ~200 bp gene sequence of Chi1 and Chi4 was encoded by the dual promoters of CaMV 35S promoter and CmGPD promoter to obtain Chi1 and Chi4 chitinase-silenced strains. Hygromycin B resistance gene (HygR) was used to screen transformants. (B,C) The amplification pattern obtained with primers for the HygR primer sets using genomic DNA isolated from the C. militaris transformants and WT. Lane P: plasmid as a positive control; Lane WT: WT strain as a negative control.