Figure 4.

Actin immunofluorescence studies. MDA-MB-231 cells were exposed for 24 h to the vehicle alone (CTRL), 0.1 μM LA or carbazole derivatives 3 and 4 (used at their IC₅₀ values). Then the cells were further processed, as indicated in the experimental section. The inverted fluorescence microscope was adopted to observe and image all the immunofluorescence figures (40× magnification). Panels A: nuclear stain with DAPI (λ_ex/λem = 350/460 nm); Panels B: β-actin (Alexa Fluor^® 568; λ_ex/λem = 644/665 nm); Panels C show a merge. Representative fields are reported.