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. 2023 Feb 23;11(3):519. doi: 10.3390/vaccines11030519

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determination of total IgG, IgG1, and IgG2a levels generated against the C. pseudotuberculosis rPknG protein on days 0 (preimmune), 21 (first immunization), and 42 (second immunization). Results are given as mean (bars) ± standard deviation of absorbance (nm) and each experimental group contained 10 animals. Different letters on the same day represent groups with statistically significant differences (p < 0.05) while the same letter shows no statistical difference (p > 0.05). All groups were compared with each other on a given day. Data from different days were not compared with each other. (A) Total anti-rPkng IgG; (B) anti-rPkng IgG1; (C) anti-rPkng IgG2a; (D) IgG2a/IgG1 anti-rPkng ratio.