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. 2023 Feb 22;15(3):608. doi: 10.3390/v15030608

Figure 2.

Figure 2

Transduction efficiency of AdV and BV in patient-derived GBM and mIDH astrocytoma neurospheres. (A) Patient-derived GBM neurospheres (NS) were incubated with AdV or BV at different MOIs, and 48 h later, transgene expression was assessed by fluorescent microscopy and quantified using software ImageJ. Representative microphotographs are shown (MOI = 750). Nuclei were stained with DAPI. (B) Correlation between the transduction efficiency of BVs in patient-derived GBM neurospheres (NS) (MOI = 750) and the expression of molecules involved in BV cell internalization: heparan sulfate proteoglycan 2 (perlecan) (HSPG2), syndecan-1 (SDC1), clathrin (CLTC), dynamin (DNM1). r: correlation coefficient (Spearman correlation test), (C) Patient-derived mIDH astrocytoma neurospheres (NS) were incubated with AdV or BV at different MOIs, and 48 h later, transgene expression was assessed by fluorescent microscopy and quantified using ImageJ. Representative microphotographs are shown (MOI = 750). Nuclei were stained with DAPI.