Figure 3.

AdV and BV transduction efficiency in rat normal and neoplastic astrocytes in vitro. C6 rat GBM cells (A) and primary cultures of normal rat astrocytes from brain cortex (B) were incubated with AdV or BV at different MOIs, and 48 h later, transgene expression was assessed by fluorescent microscopy and quantified using ImageJ. Representative microphotographs are shown (MOI = 750). Nuclei were stained with DAPI.