Figure 6.

BV-mediated transgene expression in normal and neoplastic astrocytes in vivo. (A) C57Bl/6 mice were inoculated with mouse glioma neurospheres (NS) into the brain and 3 weeks later, tumors were injected with BV (5 × 10⁸ PFU) and mice were euthanized after 5 days. The expression of the reporter protein citrine (green) in the tumor was evaluated by fluorescent microscopy. Nuclei were stained with DAPI. The insets show tumor cells with citrine expression, at higher magnification. N indicates necrotic area. (B–E) C57Bl/6 mice were inoculated into the right striatum with BV (5 × 10⁸ PFU). After 7 or 21 days, the expression of the reporter protein citrine (green) was evaluated by fluorescent microscopy. Glial cells were identified using an anti-GFAP antibody (red stain). The nuclei were stained with DAPI. Arrows indicate glial cells transduced with BV. Neuropathology was assessed by Nissl (D) and hematoxylin-eosin staining (E). V: Ventricle. CC: Corpus callosum.