Figure 7.
BV-mediated transgene expression stability in the brain of preimmunized mice. (A) C57Bl/6 mice were injected i.p with BV.dTomato (108 PFU), 30 and 15 days before administration of BV.citrine (5 × 108 PFU) into the right striatum. (B) Sera was extracted from naïve or systemically BV-preimmunized mice and the concentration of anti-BV circulating antibodies was measured by plaque reduction neutralization assay (PRNT). (C) 7 or 21 days after intracranial surgery, the expression of citrine (green) was evaluated by fluorescent microscopy and quantified using ImageJ (* p < 0.05 vs. control). Representative images of control and preimmunized mouse brains are shown. The nuclei were stained with DAPI. Arrows indicate cells transduced with BV (D).