Table 5.
Methodology of studies on sarcopenia.
| Ref. | miR | miR Assay | Tissue | Reference Genes | Technical Replicates | Timing of Sample Collection | miR Direction | Main Results |
|---|---|---|---|---|---|---|---|---|
| Chen 2019 b [32] | miR-1-3p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-100-5p, miR-125b-5p, miR-133a-3p, miR-206 | qRT-PCR | Serum | miR-16-5p, miR-93-5p, miR-191-5p |
NR | In the early morning after overnight fasting |
None | The study did not determine specific circulating miRs as biomarkers for sarcopenia |
| He 2020 [80] | miR-155, miR-208b, miR-222, miR-210, miR-328, miR-499, mir-133a, miR-133b, miR-21, miR-146a, miR-126, miR-221, and miR-20a | qRT-PCR | Plasma | cel-miR-39 | NR | After overnight fasting | ↓ miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 | miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 significantly down-regulated in sarcopenic patients compared to non-sarcopenic |
| He 2021 [81] | miR-637, miR-148a-3p, miR-125b-5p, miR-124-3p, miR-122-5p, miR-100-5p, miR-93-5p, miR-21-5p, miR-23a-3p, and miR-24-3p | qRT-PCR | Plasma | cel-miR-39 | NR | After overnight fasting | ↓ miR-23a-3p, miR-93-5p, and miR-637 | ↓ miR-23a-3p, miR-93-5p, miR-637 in the sarcopenia group than in the non-sarcopenia group |
| Liu 2021 [82] | miR-133a, miR-486, miR-21, miR-146a | qRT-PCR | Plasma | cel-miR-39-3p | NR | Fasting for at least 8 h and avoidance of strenuous physical exercise for at least 48 h |
↓ miR-486 and miR-146a | Myo-miR (miR-486) and inflammation-related miR (miR-146a) as biomarkers of age-related sarcopenia |
| Valášková 2021 [83] | miR-29a, miR-29b, miR-1, miR-133a, miR-133b, miR-206, miR-208b and miR-499 | qRT-PCR | Plasma | ce-miR-39 | NR | NR | ↑ miR-1, miR-29a and miR-29b; ↓ miR-206, miR-133a, miR-133b, miR-208b, and miR-499 |
↑ miR-1, miR-29a, and miR-29b and ↓miR-206, miR-133a, miR-133b, miR-208b, and miR-499 expression in patients with low muscle performance |
↑: increase; and ↓: decrease.