Table 1.
Putative toxin candidates with significant peptide matches from mass spectra of the SWATH-MS and DDA pooled acontia sample. Toxin families with a known function are highlighted in bold and sea anemone toxins are highlighted in italics. Sequence coverage refers to whole protein sequence and mature protein coverage refers to sequence without predicted signal peptide and cleavage sites.
| Toxin Family | Contig | Peptide Hits (Conf ≥ 95) | Sequence Coverage (%) | Mature Protein Coverage (%) |
|---|---|---|---|---|
| Disintegrin and Metalloproteinase | c59360_g1_i1 | 15 | 14.69 | 14.96 |
| Disintegrin and Metalloproteinase | c62072_g1_i1 | 10 | 13.07 | 13.40 |
| Ficolin-type lectin | c60639_g1_i1 | 3 | 10.42 | 11.11 |
| Jellyfish type II | c60596_g1_i1 | 4 | 7.60 | 7.83 |
| KTx type I (ShK) | c32422_g1_i1 | 4 | 46.59 | 59.42 |
| KTx type I (ShK) | c50551_g1_i1 | 3 | 42.16 | 52.44 |
| KTx type III | c8939_g1_i1 | 2 | 26.83 | 46.81 |
| Multicopper oxidase | c56947_g1_i1 | 5 | 21.80 | 23.39 |
| NaTx (Calitoxin) | c40761_g1_i1 | 4 | 52.17 | 91.67 |
| NaTx type I | c50240_g1_i1 | 5 | 57.89 | 100.00 |
| Peptidase M12A | c26461_g1_i1 | 7 | 11.97 | 12.40 |
| Peptidase M12A | c49341_g1_i3 | 4 | 2.47 | 2.55 |
| Peptidase M13 | c63393_g1_i1 | 13 | 5.83 | 5.99 |
| Phospholipase A2 | c56806_g1_i1 | 7 | 49.38 | 57.14 |
| Sea anemone type 8 | c47095_g1_i1 | 2 | 24.39 | 31.75 |
| Unknown | c44161_g1_i1 | 7 | 51.22 | 59.43 |
| Unknown | c56815_g1_i2 | 6 | 37.65 | 42.66 |
| Unknown | c58770_g1_i1 | 17 | 47.61 | 50.56 |