Cuticle-, chitin- and ECM-associated genes in larval and adult trachea. (A–H) Bar charts showing the Reads per kilobase of transcript per million mapped reads (RPKM) values compared between larval (blue) and adult trachea (red). In (A), genes coding for metalloproteinase; in (B), those coding for Larval Cuticle Proteins (Lcp); and in (C), those of the Ccp family are shown. (D) shows members of the Tweedle (Twdl) family, (E) those of the obstructor (obst) group, (F) members of the imaginal disc growth factor (ldgf) family, (G) those of the Chitinase (Cht) family, and (H) members of the cuticular protein (Cpr) group. Asterisks indicate a differential gene expression based on the RNA-Seq analysis. Bars show mean with SD, n = 4.