Table 6.
Compound | R | PfPK6 IC50 (nM)a | Pf3D7 blood stage EC50 (nM)b |
---|---|---|---|
41 | 13 ± 0.5 | 580 ± 65 | |
59 | 390 ± 30 | 630 ± 13 | |
60 | 135 ± 8 | Inactivec | |
61 | 310 ± 13 | 250 ± 7 | |
62 | 220 ± 40 | 270 ± 41 | |
63 | 138 ± 14 | 320 ± 13 | |
64 | 26 ± 2 | 330 ± 11 | |
65 | 150 ± 30 | 660 ± 15 | |
66 | 17 ± 3 | 330 ± 5 | |
67 | 13 ± 0.9 | 160 ± 2 | |
68 | 29 ± 3 | 140 ± 4 |
IC50 values were determined using the KinaseSeeker assay with 5-fold dilutions, presented as mean ± s.e.m. values of two experiments performed in duplicate.
EC50 values were determined using the SYBR Green I-based assay with 2-fold dilutions, presented as mean ± s.e.m. values performed in triplicate.
No inhibition when tested in triplicate at a single concentration of 1 μM.