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. 2023 Mar 28;6(6):e202301914. doi: 10.26508/lsa.202301914

Figure S3. Endogenous SLC25A46 is present at mitochondrial fusion and fission sites.

Figure S3.

(A) Immunofluorescence analysis of human fibroblasts. Endogenous SLC25A46 localization is shown in green. PRDX3 is used as a mitochondrial marker (white). Pink arrows indicate mitochondrial tips, and blue arrows indicate mitochondrial branching points. Scale bar: 5 μm. (B) Mitochondrial tips (n > 80 per condition) and branching points (n > 33 per condition) were analyzed for the positive focal SLC25A46 signal. Data are shown as the mean + SEM, n = 3. (C) Immunofluorescence analysis of cortical neurons differentiated from human iPSCs. Endogenous SLC25A46 localization is shown in green. PRDX3 is used as a mitochondrial marker (white). A zoomed image of the box area is shown on the right. Scale bar: 10 μm. (D) Immunofluorescence analysis of SLC25A46 knock-out fibroblast cell lines expressing the R257Q variant. SLC25A46 localization is shown in green. PRDX3 is used as a mitochondrial marker (white). Pink arrows indicate mitochondrial tips, and blue arrows indicate mitochondrial branching points. A zoomed image is shown on the right. Scale bar: 10 μm. (E) Mitochondrial tips (n > 60 per condition) and branching points (n > 30 per condition) were analyzed for the positive focal SLC25A46-GFP signal in fibroblasts expressing the pR257Q variant. Data are shown as the mean + SEM, n = 3.