Table 1.
Microparticles in blood. Flow cytometry was used to evaluate MPs. Total MPs/µL plasma is shown in the first column, and other columns show the percent of each that expressed proteins specific to different cells, including neutrophils (CD66b), endothelial cells (CD146), platelets (CD41a), and microglia (transmembrane protein 119, TMEM). As discussed in the text, proteins expressing TSP-1 and F-actin, evaluated as those binding phalloidin, were also assessed. Rows show values for divers in the deep group prior to their dive and after the ~100 m dive. The next three rows show results for divers in the shallow group prior to their first dive, after the first day of 3 dives, and after the last of 7 days diving. Data are mean ± SD (n = number of diver’s samples; + p < 0.001, t-test; * p < 0.001 vs. control, RM-ANOVA). Bold numbers are to indicate statistically significant values.
| MPs/µL | % CD66b | %CD146 | %TSP | %CD41 | %TMEM | %Phalloidin | |
|---|---|---|---|---|---|---|---|
| Deep-Pre (8) | 900 ± 68 | 11.0 ± 0.5 | 25.1 ± 0.5 | 12.7 ± 0.4 | 3.5 ± 0.4 | 31.3 ± 0.9 | 17.3 ± 0.2 |
| Deep-Post (8) | 1089 ± 68 + | 14.3 ± 2.0 + | 28.4 ± 1.8 + | 15.9 ± 2.3 + | 5.7 ± 1.6 + | 34.4 ± 2.1 + | 20.0 ± 1.9 + |
| Shallow-pre (6) | 799 ± 68 | 11.4 ± 2.6 | 23.0 ± 2.5 | 13.6 ± 2.5 | 3.1 ± 1.1 | 29.2 ± 2.0 | 18.4 ± 1.5 |
| Shallow- 1 day post (6) |
947 ± 31 * | 12.8 ± 1.5 | 23.6 ± 1.5 | 14.5 ± 1.4 | 4.8 ± 1.9 | 29.9 ± 0.8 | 19.4 ± 1.3 |
| Shallow- 7 day post (6) |
1016 ± 48 * | 18.7 ± 1.5 * | 27.4 ± 1.5 * | 16.2 ± 1.8 | 7.1 ± 1.8 * | 33.2 ± 1.7 * | 20.7 ± 1.5 |