Maximum-likelihood tree of the softcore-genome alignment of all P. aeruginosa isolates used in this study (n = 2009). The scale bar represents the genetic distance. Arcs in blue represent phylogroup A, yellow B, and grey C. The phylogenetic placement of the major P. aeruginosa clone type (mPact) strain panel, sequenced in this study, is highlighted in the tree, with the strain name (the “H” before each number stands for Hanover, referring to the location of the Tuemmler lab and the study that first described this collection17) next to strips coloured according to the phylogroup.