Skip to main content
. 2023 Mar 20;62:102682. doi: 10.1016/j.redox.2023.102682

Fig. 2.

Fig. 2

Regulation of ROS levels in neurons by GIT1 in vitro and in vivo. A ROS levels in the spinal cord were detected by DHE staining (scale bar = 100 μm). B Quantification of DHE fluorescence intensity in the spinal cord of Git1 WT and KO mice in the sham group and I/R group (4 h of reperfusion, n = 6). C Cell immunofluorescence detection of ROS levels in neurons (scale bar = 50 μm). D Quantification of mean fluorescence intensity of ROS in neurons (n = 6). E,F Immunofluorescence detection and quantification of mitochondrial superoxide (MitoSox, red) in neurons (scale bar = 50 μm, n = 6). G,H Immunofluorescence detection and quantification of lipid peroxidation (green) in neurons (scale bar = 50 μm, n = 6) (OGD/R, reperfusion 4 h). *P < 0.05; **P < 0.01; ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)