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. 2023 Mar 20;62:102682. doi: 10.1016/j.redox.2023.102682

Fig. 3.

Fig. 3

Git1 regulates endoplasmic reticulum stress and DNA damage in neurons after I/R and OGD/R. A–C Western blotting analysis of the expression of ERS (phosphorylated eIF-2α) and DNA damage (phosphorylated H2A.X)-related proteins in spinal cord after I/R (reperfusion 4 h) (n = 3). D, E Immunofluorescence analysis shows ERS levels (phosphorylated eIF-2α) in neurons in the spinal cord (scale bar = 100 μm, n = 3) (I/R, reperfusion 4 h). F, G Immunofluorescence analysis shows DNA damage levels (phosphorylated H2A.X) in neurons in the spinal cord (scale bar = 100 μm, n = 3). H–J Western blot analysis of the levels of ERS (phosphorylated eIF-2α) and DNA damage (phosphorylated H2A.X)-related proteins in neurons after OGD/R or NAC treatment (with OGD/R) (n = 3). K–N Immunofluorescence of phosphorylated eIF-2α and H2A.X expression levels in neurons after OGD/R or NAC treatment (during reperfusion) (scale bar = 20 μm, n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.