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. 2023 Mar 20;62:102682. doi: 10.1016/j.redox.2023.102682

Fig. 4.

Fig. 4

Git1 interacts with PGK1. A Interaction between Git1 and other proteins. B, C Co-immunoprecipitation detection of endogenous Git1 binding to PGK1 in untreated or OGD/R-treated neurons and 293T cells (OGD/R reoxygenation for 4 h, n = 3). D Proximity ligation assay (PLA) detects the binding of Git1 to PGK1 in neurons under physiologic conditions or in response to OGD/R treatment (reperfusion 4 h) (scale bar = 50 μm, n = 6). E Functional domains of Git1. F Flag-Git1-WT or Flag-Git1 deletion mutants were co-transfected with HA-PGK1 in HEK 293T cells, and were then subjected to OGD/R for 4 h. Immunoprecipitation (IP) was conducted using Flag antibody, followed by probing with HA to detect the interaction between PGK1 and Git1 or Git1 deletion mutants (n = 3). G Functional domains of PGK1. H HEK 293T cells were co-transfected with Flag-Git1 and either full-length HA-labeled PGK1 or its deletion mutant. The cell lysate was subsequently analyzed by IP (n = 3). I Three-dimensional structures of Git1 and PGK1 proteins.