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. 2023 Mar 20;62:102682. doi: 10.1016/j.redox.2023.102682

Fig. 7.

Fig. 7

Overexpression of Git1 reduces reperfusion-induced ROS levels in neurons. A Detection of neuronal ROS levels in spinal cord by immunofluorescence staining (scale bar = 100 μm). B Quantification of DHE fluorescence intensity in the spinal cord of Git1 Vec and Git1 OE mice after sham-operated and I/R (4 h of reperfusion, n = 6). C, D Representative immunofluorescence images of ROS levels in Git1 Vec and Git1 OE neurons and quantification of mean fluorescence intensity of ROS (scale bar = 50 μm, n = 6). E, F Immunofluorescence and quantification of mitochondrial superoxide (MitoSox labeled, red) in Git1 Vec and Git1 OE neurons (scale bar = 50 μm, n = 6). G, H Immunofluorescence detection and quantification of lipid peroxidation (green) in Git1 Vec and Git1 OE neurons (scale bar = 50 μm, n = 6). (OGD/R, reperfusion 4 h). *P < 0.05; **P < 0.01; ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)