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. 2023 Mar 20;62:102682. doi: 10.1016/j.redox.2023.102682

Fig. 8.

Fig. 8

Overexpression of Git1 in neurons enhances Keap1-Nrf2 signaling pathway. A Western blot analysis of Keap1 and HMM-Keap1 expression in the spinal cords of Git1 Vec and OE mice following either a sham operation or ischemia/reperfusion (I/R) injury for 4 h. Keap1: black dotted line box, HMM-Keap1: black solid line box. B Quantification of HMM-Keap1 in the spinal cord (n = 3). C Representative western blots of Keap1 and HMM-Keap1 expression in Git1 Vec and OE neurons under control and OGD/R treatment (reperfusion 4 h). Keap1: black dotted line box, HMM-Keap1: black solid line box. D Quantification of phosphorylation of HMM-Keap1 in neurons (n = 3). E, F Representative western blots of Nrf2, HO1, NQO1, and thioredoxin 1 expression levels in the spinal cords of Git1 Vec and OE mice subjected to sham operation or ischemia/reperfusion (I/R) injury for 4 h (n = 3). G-I Representative western blots of Nrf2 (total), Nrf2 (nuclear), HO1, NQO1, and thioredoxin 1 expression in Git1 Vec and OE neurons under control and OGD/R treatment (reperfusion 4 h) (n = 3). J, K Representative immunofluorescence images of ROS levels in AAV-Vec (Nrf2 vector adenovirus) and AAV-Nrf2 (Nrf2 knockdown adenovirus) Git1 OE-neurons and quantification of mean fluorescence intensity of ROS (scale bar = 50 μm, n = 6). *P < 0.05; **P < 0.01; ***P < 0.001.