Fig. 3.

Effect of HCA2 on inflammatory responses in the mice. A–C The expression of pro-inflammatory mediators in mouse midbrain and serum, as detected using the ELISA method. D The mRNA expression of pro-inflammatory protease (iNOS and COX2), as detected using real-time PCR. E Pathway enrichment of differential genes between WT mice and HCA2^−/− mice in midbrain after LPS injection. F GSEA enrichment map of differential genes between WT mice and HCA2^−/− mice in midbrain after LPS injection. G Network interaction map of differential genes and pathways between WT mice and HCA2^−/− mice in midbrain after LPS injection. Results are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01 means a significant difference and ns means not significant