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Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1994 Jun;53(6):391–395. doi: 10.1136/ard.53.6.391

Increased IgA antibodies to cytokeratins in the spondyloarthropathies.

A A Borg 1, N B Nixon 1, P T Dawes 1, D L Mattey 1
PMCID: PMC1005354  PMID: 7518663

Abstract

OBJECTIVES--Increased levels of IgA antibodies to cytokeratin-18 (CK-18) and epidermal keratins (EpK) in the sera of patients with rheumatoid arthritis (RA) have been demonstrated previously. In the present study investigations were carried out to determine whether levels of these autoantibodies were also raised in the spondyloarthropathies, and whether there was any association with particular disease manifestations. METHODS--Using specific enzyme linked immunosorbent assays (ELISA) measurements were taken of IgA, IgG and IgM antibodies to EpK and to CK-18 in the sera of patients with psoriatic arthropathy, ankylosing spondylitis (AS), Reiter's syndrome, psoriasis and in normal subjects. RESULTS--IgA antibodies to both EpK and CK-18 were significantly increased in sera from patients with psoriasis and psoriatic arthropathy but not in the sera from the patients with AS or Reiter's syndrome, or in the controls. In psoriatic arthritis, however, these levels were significantly higher only in those patients with peripheral joint disease and not in those with axial arthritis alone. There was no significant increase in antibody levels in patients with AS or Reiter's syndrome. There were no differences in the levels of IgG or IgM antibodies to CK-18 or EpK between the patient groups and controls. CONCLUSIONS--Raised levels of IgA antibodies to CK-18 and EpK in psoriatic arthropathy and psoriasis probably reflect exposure of intracellular cytokeratin antigens to the immune system after damage to cytokeratin containing cells, and suggests a common pathogenic mechanism in these conditions which involves production of cytokeratin autoantibodies. In patients with psoriatic arthropathy, such a mechanism appears only to be operating in patients with peripheral joint involvement and not in those with axial arthritis.

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Selected References

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  1. Armstrong R. D., Panayi G. S., Welsh K. I. Histocompatibility antigens in psoriasis, psoriatic arthropathy, and ankylosing spondylitis. Ann Rheum Dis. 1983 Apr;42(2):142–146. doi: 10.1136/ard.42.2.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baboonian C., Venables P. J., Williams D. G., Williams R. O., Maini R. N. Cross reaction of antibodies to a glycine/alanine repeat sequence of Epstein-Barr virus nuclear antigen-1 with collagen, cytokeratin, and actin. Ann Rheum Dis. 1991 Nov;50(11):772–775. doi: 10.1136/ard.50.11.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barry R. E., Salmon P. R., Read A. E., Warin R. P. Mucosal architecture of the small bowel in cases of psoriasis. Gut. 1971 Nov;12(11):873–877. doi: 10.1136/gut.12.11.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birkenfeld P., Haratz N., Klein G., Sulitzeanu D. Cross-reactivity between the EBNA-1 p107 peptide, collagen, and keratin: implications for the pathogenesis of rheumatoid arthritis. Clin Immunol Immunopathol. 1990 Jan;54(1):14–25. doi: 10.1016/0090-1229(90)90002-8. [DOI] [PubMed] [Google Scholar]
  5. Calguneri M., Swinburne L., Shinebaum R., Cooke E. M., Wright V. Secretory IgA: immune defence pattern in ankylosing spondylitis and klebsiella. Ann Rheum Dis. 1981 Dec;40(6):600–604. doi: 10.1136/ard.40.6.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cowling P., Ebringer R., Ebringer A. Association of inflammation with raised serum IgA in ankylosing spondylitis. Ann Rheum Dis. 1980 Dec;39(6):545–549. doi: 10.1136/ard.39.6.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Espinoza L. R., Vasey F. B., Oh J. H., Wilkinson R., Osterland C. K. Association between HLA-BW38 and peripheral psoriatic arthritis. Arthritis Rheum. 1978 Jan-Feb;21(1):72–75. doi: 10.1002/art.1780210112. [DOI] [PubMed] [Google Scholar]
  8. Franke W. W., Moll R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 1987;36(2):145–163. doi: 10.1111/j.1432-0436.1987.tb00189.x. [DOI] [PubMed] [Google Scholar]
  9. Heid H. W., Moll I., Franke W. W. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 1988;37(2):137–157. doi: 10.1111/j.1432-0436.1988.tb00805.x. [DOI] [PubMed] [Google Scholar]
  10. Hocini H., Iscaki S., Benlahrache C., Vitalis L., Chevalier X., Larget-Piet B., Bouvet J. P. Increased levels of serum IgA as IgA1 monomers in ankylosing spondylitis. Ann Rheum Dis. 1992 Jun;51(6):790–792. doi: 10.1136/ard.51.6.790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jahn L., Fouquet B., Rohe K., Franke W. W. Cytokeratins in certain endothelial and smooth muscle cells of two taxonomically distant vertebrate species, Xenopus laevis and man. Differentiation. 1987;36(3):234–254. doi: 10.1111/j.1432-0436.1987.tb00198.x. [DOI] [PubMed] [Google Scholar]
  12. Jurik A. G., Graudal H., Kirstein H. Lack of antikeratin antibodies in patients with palmoplantar pustular eruptions and arthropathy. Arch Dermatol Res. 1989;281(3):185–187. doi: 10.1007/BF00456390. [DOI] [PubMed] [Google Scholar]
  13. Lane E. B., Bártek J., Purkis P. E., Leigh I. M. Keratin antigens in differentiating skin. Ann N Y Acad Sci. 1985;455:241–258. doi: 10.1111/j.1749-6632.1985.tb50415.x. [DOI] [PubMed] [Google Scholar]
  14. Marks J., Shuster S. Small-intestinal mucosal abnormalities in various skin diseases--fact or fancy? Gut. 1970 Apr;11(4):281–291. doi: 10.1136/gut.11.4.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mattey D. L., Nixon N., Wynn-Jones C., Dawes P. T. Demonstration of cytokeratin in endothelial cells of the synovial microvasculature in situ and in vitro. Br J Rheumatol. 1993 Aug;32(8):676–682. doi: 10.1093/rheumatology/32.8.676. [DOI] [PubMed] [Google Scholar]
  16. Metzger A. L., Morris R. I., Bluestone R., Terasaki P. I. HL-A W27 in psoriatic arthropathy. Arthritis Rheum. 1975 Mar-Apr;18(2):111–115. doi: 10.1002/art.1780180203. [DOI] [PubMed] [Google Scholar]
  17. Moll J. M., Wright V. Psoriatic arthritis. Semin Arthritis Rheum. 1973;3(1):55–78. doi: 10.1016/0049-0172(73)90035-8. [DOI] [PubMed] [Google Scholar]
  18. Moll R., Moll I., Franke W. W. Identification of Merkel cells in human skin by specific cytokeratin antibodies: changes of cell density and distribution in fetal and adult plantar epidermis. Differentiation. 1984;28(2):136–154. doi: 10.1111/j.1432-0436.1984.tb00277.x. [DOI] [PubMed] [Google Scholar]
  19. Murray C., Mann D. L., Gerber L. N., Barth W., Perlmann S., Decker J. L., Nigra T. P. Histocompatibility alloantigens in psoriasis and psoriatic arthritis. Evidence for the influence of multiple genes in the major histocompatibility complex. J Clin Invest. 1980 Oct;66(4):670–675. doi: 10.1172/JCI109903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roux H., Mercier P., Maestracci D., Serratrice G., Sany J., Seignalet J., Serre H. Psoriatic arthritis and HLA antigens. J Rheumatol Suppl. 1977;3:64–65. [PubMed] [Google Scholar]
  21. Senécal J. L., Ichiki S., Girard D., Raymond Y. Autoantibodies to nuclear lamins and to intermediate filament proteins: natural, pathologic or pathogenic? J Rheumatol. 1993 Feb;20(2):211–219. [PubMed] [Google Scholar]
  22. Simenon G., Van Gossum A., Adler M., Rickaert F., Appelboom T. Macroscopic and microscopic gut lesions in seronegative spondyloarthropathies. J Rheumatol. 1990 Nov;17(11):1491–1494. [PubMed] [Google Scholar]
  23. Simon M., Girbal E., Sebbag M., Gomès-Daudrix V., Vincent C., Salama G., Serre G. The cytokeratin filament-aggregating protein filaggrin is the target of the so-called "antikeratin antibodies," autoantibodies specific for rheumatoid arthritis. J Clin Invest. 1993 Sep;92(3):1387–1393. doi: 10.1172/JCI116713. [DOI] [PMC free article] [PubMed] [Google Scholar]

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