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. 1994 Aug;53(8):535–539. doi: 10.1136/ard.53.8.535

Yersinia-specific antibodies in serum and synovial fluid in patients with Yersinia triggered reactive arthritis.

O Mäki-Ikola 1, R Lahesmaa 1, J Heesemann 1, R Merilahti-Palo 1, R Saario 1, A Toivanen 1, K Granfors 1
PMCID: PMC1005395  PMID: 7944640

Abstract

OBJECTIVES--To further evaluate the role of bacterial antigens in triggering inflammation in the joint in patients with reactive arthritis by studying local antibody synthesis in the joint. METHODS--Yersinia-specific antibodies in paired serum and synovial fluid samples from 29 patients with yersinia triggered reactive arthritis were studied using an enzyme linked immunosorbent assay (ELISA), an inhibition ELISA with six monoclonal antibodies against lipopolysaccharide or released proteins of yersinia and immunoblotting. Antibodies of IgM, IgG and IgA classes, as well as antibodies of IgA subclasses and those containing secretory component were measured against the lipopolysaccharide and the sodium dodecyl sulphate extract of whole Yersinia enterocolitica O:3 bacteria. RESULTS--It was shown that yersinia-specific antibodies, as well as antibodies against other microbial antigens (rubella, measles, Bordetella pertussis, tetanus toxoid and Candida albicans) in synovial fluid mirror those in serum by concentration, by specificity and by distribution in classes and subclasses. CONCLUSION--These results do not suggest any strong local antibody production, but indicate that the majority of yersinia antibodies in the synovial fluid are derived from the circulation.

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Selected References

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  1. Granfors K. Do bacterial antigens cause reactive arthritis? Rheum Dis Clin North Am. 1992 Feb;18(1):37–48. [PubMed] [Google Scholar]
  2. Granfors K., Jalkanen S., von Essen R., Lahesmaa-Rantala R., Isomäki O., Pekkola-Heino K., Merilahti-Palo R., Saario R., Isomäki H., Toivanen A. Yersinia antigens in synovial-fluid cells from patients with reactive arthritis. N Engl J Med. 1989 Jan 26;320(4):216–221. doi: 10.1056/NEJM198901263200404. [DOI] [PubMed] [Google Scholar]
  3. Granfors K., Ogasawara M., Hill J. L., Lahesmaa-Rantala R., Toivanen A., Yu D. T. Analysis of IgA antibodies to lipopolysaccharide in Yersinia-triggered reactive arthritis. J Infect Dis. 1989 Jun;159(6):1142–1147. doi: 10.1093/infdis/159.6.1142. [DOI] [PubMed] [Google Scholar]
  4. Granfors K., Toivanen A. IgA-anti-yersinia antibodies in yersinia triggered reactive arthritis. Ann Rheum Dis. 1986 Jul;45(7):561–565. doi: 10.1136/ard.45.7.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hammer M., Zeidler H., Klimsa S., Heesemann J. Yersinia enterocolitica in the synovial membrane of patients with Yersinia-induced arthritis. Arthritis Rheum. 1990 Dec;33(12):1795–1800. doi: 10.1002/art.1780331206. [DOI] [PubMed] [Google Scholar]
  6. Heesemann J., Gross U., Schmidt N., Laufs R. Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media. Infect Immun. 1986 Nov;54(2):561–567. doi: 10.1128/iai.54.2.561-567.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoogkamp-Korstanje J. A., de Koning J., Heesemann J. Persistence of Yersinia enterocolitica in man. Infection. 1988 Mar-Apr;16(2):81–85. doi: 10.1007/BF01644307. [DOI] [PubMed] [Google Scholar]
  8. Inman R. D., Johnston M. E., Klein M. H. Analysis of serum and synovial fluid IgA in Reiter's syndrome and reactive arthritis. Clin Immunol Immunopathol. 1987 May;43(2):195–203. doi: 10.1016/0090-1229(87)90127-9. [DOI] [PubMed] [Google Scholar]
  9. Kaukonen K., Savolainen J., Viander M., Terho E. O. Characterization of Aspergillus umbrosus carbohydrate antigens by biotinylated lectins and IgG response to mannan/mannoprotein antigens in patients with farmer's lung. Clin Exp Allergy. 1993 Jan;23(1):21–27. doi: 10.1111/j.1365-2222.1993.tb02479.x. [DOI] [PubMed] [Google Scholar]
  10. Kett K., Brandtzaeg P., Fausa O. J-chain expression is more prominent in immunoglobulin A2 than in immunoglobulin A1 colonic immunocytes and is decreased in both subclasses associated with inflammatory bowel disease. Gastroenterology. 1988 Jun;94(6):1419–1425. doi: 10.1016/0016-5085(88)90681-6. [DOI] [PubMed] [Google Scholar]
  11. Lahesmaa-Rantala R., Granfors K., Isomäki H., Toivanen A. Yersinia specific immune complexes in the synovial fluid of patients with yersinia triggered reactive arthritis. Ann Rheum Dis. 1987 Jul;46(7):510–514. doi: 10.1136/ard.46.7.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lahesmaa-Rantala R., Heesemann J., Lehtonen O. P., Granfors K., Toivanen A. Avidity of antibodies against released proteins of Yersinia spp: comparison of patients with or without reactive arthritis. Ann Rheum Dis. 1989 Dec;48(12):1003–1006. doi: 10.1136/ard.48.12.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lahesmaa R., Yssel H., Batsford S., Luukkainen R., Möttönen T., Steinman L., Peltz G. Yersinia enterocolitica activates a T helper type 1-like T cell subset in reactive arthritis. J Immunol. 1992 May 15;148(10):3079–3085. [PubMed] [Google Scholar]
  14. Musiani M., Zerbini M., Ferri S., Plazzi M., Gentilomi G., La Placa M. Comparison of the immune response to Epstein-Barr virus and cytomegalovirus in sera and synovial fluids of patients with rheumatoid arthritis. Ann Rheum Dis. 1987 Nov;46(11):837–842. doi: 10.1136/ard.46.11.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mäki-Ikola O., Granfors K. Salmonella-triggered reactive arthritis. Lancet. 1992 May 2;339(8801):1096–1098. doi: 10.1016/0140-6736(92)90675-s. [DOI] [PubMed] [Google Scholar]
  16. Mäki-Ikola O., Leirisalo-Repo M., Kantele A., Toivanen P., Granfors K. Salmonella-specific antibodies in reactive arthritis. J Infect Dis. 1991 Dec;164(6):1141–1148. doi: 10.1093/infdis/164.6.1141. [DOI] [PubMed] [Google Scholar]
  17. Mäki-Ikola O., Pulz M., Heesemann J., Lahesmaa R., Saario R., Toivanen A., Granfors K. Antibody response against 26 and 46 kilodalton released proteins of yersinia in yersinia triggered reactive arthritis. Ann Rheum Dis. 1992 Nov;51(11):1247–1249. doi: 10.1136/ard.51.11.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mäki-Ikola O., Yli-Kerttula U., Saario R., Toivanen P., Granfors K. Salmonella specific antibodies in serum and synovial fluid in patients with reactive arthritis. Br J Rheumatol. 1992 Jan;31(1):25–29. doi: 10.1093/rheumatology/31.1.25. [DOI] [PubMed] [Google Scholar]
  19. Salmi M., Granfors K., Leirisalo-Repo M., Hämäläinen M., MacDermott R., Leino R., Havia T., Jalkanen S. Selective endothelial binding of interleukin-2-dependent human T-cell lines derived from different tissues. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11436–11440. doi: 10.1073/pnas.89.23.11436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Salonen J., Vainionpä R., Halonen P. Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens. Arch Virol. 1986;91(1-2):93–106. doi: 10.1007/BF01316731. [DOI] [PubMed] [Google Scholar]
  21. Sieper J., Braun J., Brandt J., Miksits K., Heesemann J., Laitko S., Sörensen H., Distler A., Kingsley G. Pathogenetic role of Chlamydia, Yersinia and Borrelia in undifferentiated oligoarthritis. J Rheumatol. 1992 Aug;19(8):1236–1242. [PubMed] [Google Scholar]

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