Figure 10.

Indirect MTT cytotoxicity assay on NHDF (a), and HSF (b); Biofiber extracts (0.25, 1, 2.25 cm²) in DMEM (2 mL) at 37 °C for 24–72 h were used as the treatment solution. Untreated cells have been used as positive (CTR+) while, treated with phenol as negative control (CTR−). The viability threshold was fixed at 70% (red dot line) according to ISO 10993-12. The dressing safety was confirmed at 72 h of treatment, with live/dead staining (c). The observation will be performed with ViCo semi-confocal microscope (20×), whose function is managed by IMAJE PRO 6.2 software (Houston, TX USA). Some standard deviations are not noticeable as <10.