KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| 20x NuPAGE MES Puffer | Invitrogen | NP0002 |
| 2-Mercapthoethanol | GIBCO | 3130010 |
| 4x NuPage LDS loading buffer | Invitrogen | NP0007 |
| ACK lysis buffer | GIBCO | A1049201 |
| Alexa Fluor 647-conjugated Cholera Toxin B | Life Technologies | C34778 |
| anti-CD14+ coated beads | Miltenyi Biotech | 130-050-201 |
| Bafilomcyin A1 | Abcam | ab120497 |
| Bovine serum albumin | AppliChem | A1391.0500 |
| Calcofluor white | Sigma-Aldrich | 18909 |
| Calmodulin inhibitor W7 | Sigma-Aldrich | A3281 |
| CheLuminate-HRP PicoDetect | AppliChem | A3417,1200 |
| Cholesterol | Sigma-Aldrich | C8667 |
| DMEM | GIBCO | 11960044 |
| DMSO | VWR | 1029500500 |
| Fetal bovine serum | GE Healthcare Life Sciences | SV30160.03. |
| Fluo-4 | Life Technologies | F10473 |
| Fluorescein isothiocyanate | Sigma-Aldrich | F7250 |
| Gentamicin sulfate | GIBCO | 15750060 |
| GM-CSF | Miltenyi Biotec | 130-093-862 |
| HEPES (1M) | GIBCO | 15630056 |
| Histopaque®-1077 | Sigma-Aldrich | 10771 |
| Human serum | Sigma-Aldrich | H3667 |
| Lipofectamin 2000 | Life Technologies | 11668019 |
| L-Leucine methyl ester hydrochloride (LLOMe) | Sigma-Aldrich | L1002 |
| LysoTracker Red DND-99 | Life Technologies | L7528 |
| M-CSF | Peprotech | 315-02 |
| Methyl-β-cyclodextrin | Sigma-Aldrich | C4555 |
| Normal goat serum | Invitrogen | 01-6201 |
| N-pentadecanoyl-D-ejyfhro-sphingosine | Biotrend | 2037 |
| pHRodo | Invitrogen | P36011 |
| RPMI-1640 | GIBCO | 21875034 |
| siRNA | Santa Cruz Biotechnology | N/A |
| Sodium bicarbonate | GIBCO | 25080094 |
| Sodium dodecyl sulfate | Merck | 817034 |
| Ultraglutamine | GIBCO | 35050061 |
| Antibodies | ||
| primary | ||
| Rabbit anti-ATP6V1B2 | Abcam | ab73404; RRID:AB_1924799 |
| Rabbit anti-p47phox | Santa Cruz Biotechnology | sc-14015; RRID:AB_2150289 |
| Rabbit anti-Flotillin-1 | Santa Cruz Biotechnology | sc-25506; RRID:AB_2106567 |
| Goat anti-Flotillin-1 | Abcam | ab13493; RRID:AB_2294271 |
| Mouse anti-Flotillin-1 | BD Biosciences | 610820; RRID:AB_398139 |
| Mouse anti-Flotillin-2 | BD Biosciences | 610384; RRID:AB_610384 |
| Rabbit anti-Caveolin-1 | Cell Signaling Technology | 3267; RRID:AB_2275453 |
| Rabbit anti-Caveolin-2 | Abcam | ab133484 |
| Rabbit anti-GAPDH | Cell Signaling Technology | 2118; RRID:AB_561053 |
| Rabbit anti-CHMP4B | Abcam | ab105767; RRID:AB_10858466 |
| Rabbit anti-Galectin 3 | Santa Cruz Biotechnology | sc-515582 |
| secondary | ||
| Goat anti-rabbit Alexa Fluor 647 | Cell Signaling Technology | 4414; RRID:AB_10693544 |
| Goat anti-rabbit IgG DyLight 633 | Thermo Fisher Scientific | 35562; RRID:AB_1307539 |
| Goat anti-rabbit Abberior Star Red | Abberior GmbH | STRED; RRID:AB_2833015 |
| Goat anti-rabbit Alexa Fluor 555 | Life Technologies | A-21430; RRID:AB_10374475 |
| Donkey anti-goat Alexa Fluor 488 | Life Technologies | A-11055; RRID:AB_2534102 |
| Goat anti-rabbit IgG-HRP | Santa Cruz Biotechnology | sc-2054; RRID:AB_631748 |
| anti-mouse IgG-HRP | Cell Signaling Technology | 7076; RRID:AB_330924 |
| Critical Commercial Assays | ||
| CellTiter-Blue® Cell Viability assay | Promega | G8080 |
| Qproteome Cell Compartment Kit | QIAGEN | 37502 |
| 2-D Quant Kit | GE Healthcare | 80648356 |
| ELISA MAX™ Deluxe Set Human IL-1β | BioLegend | 437004 |
| ELISA MAX™ Deluxe Set Human IL-6 | BioLegend | 430504 |
| ELISA MAX™ Deluxe Set Human IL-10 | BioLegend | 430604 |
| ELISA MAX™ Deluxe Set Human TNFα | BioLegend | 430204 |
| KASPar assay | LGC Genomics | https://www.biosearchtech.com |
| Experimental Models: Cell lines | ||
| MH-S | ATCC | ATCC:CRL-2019 |
| J774A.1 | ATCC | ATCC:TIB-67 |
| HeLa | ATCC | ATCC:CCL-2 |
| RAW264.7 | ATCC | ATCC:TIB-71 |
| Experimental Models: Organisms/Strains | ||
| Aspergillus fumigatus strain ATTCC46645 | ATCC | ATCC:46645 |
| Aspergillus fumigatus strain ATTCC46645 pksP | Jena Microbial Resource Collection (JMRC), HKI Jena | (Langfelder et al., 1998) |
| Flot−/− mice (male) | Ben J. Nichols (Cambridge, UK) | (Bitsikas et al., 2014) |
| C57BL/6J mice (male) | Service Unit Experimental Biomedicine of the University Hospital Jena, Jena, Germany | N/A |
| Software | ||
| Custom-written Fiji macro, based on the ImageJ version 1.51w | N/A | https://fiji.sc; (Schindelin et al., 2012) |
| Imaris 8.4.1 | Bitplane AG | https://imaris.oxinst.com |
| Quantity One | Bio-Rad | https://www.bio-rad.com |
| Analyst 1.4 | Sciex | https://sciex.com |
| Zeiss ZEN software 2.3 | Zeiss | http://www.zeiss.de/ |