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. 2023 Mar 21;15(6):1518. doi: 10.3390/nu15061518

Figure 2.

Figure 2

Effects of CSR and SLAB51 on protein and lipid oxidation. (A). Quantification of protein carbonyls, nitrotyrosine, dityrosine, and lipid 4-HNE adduct in brain homogenates in all groups of mice. The densitometric analyses obtained from three separate blots. Values are expressed as mean ± standard error. One-way ANOVA results were (F (3, 24) = 9.277; p = 0.0003) for carbonyls, (F (3, 24) = 7.550; p = 0.001) for nitrotyrosine, (F (3, 24) = 5.431; p = 0.00539) for dityrosine, and (F (3, 24) = 3.967; p = 0.01183) for lipid 4-HNE. * indicates statistical significance between S-w and CSR-w, while # indicates statistical significance between CSR-w and CSR-p. (B). Representative immunoblots for protein carbonyls, nitrotyrosine, dityrosine, and lipid 4-HNE adduct. (C). Equal protein loading for nitrotyrosine, dityrosine, and 4-HNE adducts were verified by using an anti-GAPDH antibody. Ponceau staining has been used to check loading in oxyblot. Molecular weight standards (6–205 kDa) were used for molar mass calibration.