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. Author manuscript; available in PMC: 2023 Mar 29.
Published in final edited form as: Science. 2022 Jun 10;376(6598):eabm9326. doi: 10.1126/science.abm9326

Fig. 3. Interactions mediated by Nup205 and the Nup214-Nup88-Nup62 complex.

Fig. 3.

(A) Overall interactions of inner Nup205 (orange) and outer Nup205 (yellow) with Y-complexes. Outer Nup205 directly interacts with Nup160, Nup85, and Seh1 of the outer Y-complex and with Nup43 of the inner Y-complex. The inner Nup205 directly interacts with Nup160 of the inner Y-complex, C-terminal region of Nup155, and Nup88 β-propeller in the Nup214-Nup88-Nup62 complexes. The dashed arrows indicate the locational difference between inner and outer Nup205. (B) Superposition of the inner (blue) and outer (green) Y-complexes together with the bound Nup205 molecules, showing the positions of the inner (orange) and outer (yellow) Nup205 relative to Nup85 and Nup160. The N-terminal region of Nup205 binds similarly to inner and outer Nup160 molecules; the C-terminal domain binds outer Nup85 but pivots away from the inner Nup85 because of the presence of the Nup214-Nup88-Nup62 complexes. (C) Overview (left) and fitting (right) of the AlphaFold-predicted one Nup214-Nup88-Nup62 complex into the cryo-EM density map of NPC CR monomer (contour level, 4.5 σ). (D) Overview (left) and fitting (right) of the AlphaFold-predicted Nup214-Nup88-Nup62 complexes into the cryo-EM density map (contour level, 4.5 σ), with the neighboring inner Nup85. Two Nup214-Nup88-Nup62 complexes are shown.