Figure 2.

Immunoblot analysis of L1 proteins in transplastomic lines. (A) Extracts totaling 4 µg from transplastomic L1-HPV18.2 and LTB::L1-HPV16B plants were analyzed by immunoblotting using the MD2H11 monoclonal antibody. Proteins from RV lines and wt served as negative controls. (B) Quantification of L1-HPV18.2 protein accumulation in transplastomic lines. Protein extraction was performed from total plant leaf tissue from plants belonging to two independent L1-HPV18.2 transplastomic lines grown for four weeks on 0.5 MS medium with sucrose. For both lines #27 and #28, five µg of total plant protein extracts was analyzed by SDS-PAGE and blotted onto a nylon membrane. The MD2H11 monoclonal antibody directed against the L1 protein was used to probe the blot. For quantification purposes, different amounts of purified recombinant HPV18 L1 protein were loaded onto the same gel.