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[Preprint]. 2023 Mar 13:2023.03.13.532278. [Version 1] doi: 10.1101/2023.03.13.532278

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Figure 2. — LC-ESI-MS analysis of HlyU in vitro reactivity upon the addition of GSSH or H₂O₂ (A) HlyU reacts with IAM but not with dimedone, showing that the protein is fully reduced. (B) HlyU reacts with GSSH to form a tetrasulfide link between its two cysteines, as seen in the mass shift of +62, corresponding to the addition of two sulfur atoms and the subtraction of two hydrogens. (C) HlyU does not react with H₂O₂, as seen by the absence of a peak corresponding to a dimedone adduct (115).