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[Preprint]. 2023 Mar 14:2023.03.13.532469. [Version 1] doi: 10.1101/2023.03.13.532469

Figure 3. Strong aversive experience drives co-reactivation of the Neutral ensemble with the Neutral ∩ Aversive ensemble.

Figure 3.

A) Schematic of experimental design, as in Figure 2A, but now focusing only on OfflineDay2, following Aversive learning.

B) Representation of the quantification of independent or coincident burst participation. Burst events were defined by the whole recorded population, as in Figure 2E. However, now the z-scored mean population activity of the Aversive and Neutral ensembles was computed to ask how frequently either ensemble or both ensembles participated in bursts.

C) Only in High Shock mice, the Neutral ensemble participated independently in a larger fraction of events than the Aversive ensemble did. The Aversive and Neutral ensembles participated coincidentally no differently in Low vs High shock mice. Significant main effect of Ensemble (F2,26 = 22.71, p = 0.0000002) and significant interaction between Ensemble & Amplitude (F2,26 = 5.64, p = 0.009). Post-hoc tests demonstrate significantly higher Neutral vs Aversive independent participation in High Shock mice (t7 = 6.04, p = 0.0016). No difference in co-participation between Low vs High Shock mice (t8.69 = 1.65, p = 0.13), and a trend toward lower independent participation of the Neutral ensemble in Low vs High Shock mice (t11.16 = 2.23, p = 0.095) and a trend toward higher independent participation of the Aversive ensemble in Low vs High Shock (t11.6 = 2.05, p = 0.095).

D) Burst events were now defined instead of on the entire population, now based on the mean activity of each ensemble reaching above threshold, to ask how frequently the Neutral ∩ Aversive ensemble co-bursted with either the Neutral ensemble or the Aversive ensemble.

E) In High Shock mice, the Neutral ∩ Aversive ensemble co-bursted with the Neutral ensemble more than with the Aversive ensemble. In Low Shock mice, the Neutral ∩ Aversive ensemble co-bursted no differently with the two ensembles. Significant interaction between Ensemble & Amplitude (F1,13 = 11.68, p = 0.0046). Post-hoc tests demonstrate higher co-participation with the Neutral ensemble in High Shock mice (t7 = 4.398, p = 0.006) and not in Low Shock mice (t6 = 0.940, p = 0.383).