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[Preprint]. 2023 Oct 2:2023.03.13.531968. Originally published 2023 Mar 15. [Version 2] doi: 10.1101/2023.03.13.531968

Figure 3: Δ9-THC exposure provokes an increase in glycolytic rates in ESCs and EpiLCs.

Figure 3:

(A) Diagram illustrating Δ9-THC exposure scheme and experimental strategy. (B) The NAD(P)+/NADPH ratio of stem cells exposed to the different Δ9-THC doses was normalized to the one measured in the mock-treated condition. Median and associated errors were plotted in whisker boxplots. (C) Mean fluorescence intensity (MFI) associated with the Mitotracker CMXRos stain was normalized to the one measured in the mock-treated condition. Median and associated errors were plotted in whisker boxplots. (D) Median and associated error of the maximal extracellular acidification rate (ECAR) measured in cells exposed to the different Δ9-THC doses and normalized to the protein content was plotted in whisker boxplots. (E) Median and associated error of the maximal oxygen consumption rate (OCR) measured in cells exposed to the different Δ9-THC doses and normalized to the protein content was plotted in whisker boxplots. For (B and C), 5 technical repeats of 3 biological repeats (n=15) were plotted. One same representative experiment out of three independent experiments was used to plot results in (D and E). Statistical significance: *(p<0.05), **(p<0.01), ***(p<0.001), ****(p<0.0001).