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[Preprint]. 2023 Mar 21:2023.02.11.528154. [Version 2] doi: 10.1101/2023.02.11.528154

Table 1.

SPOP variant (residues 28 – 359) Isodesmic KDa [μM] KD for peptide [μM]b KD for cDAXX [μM]c
WT 2.7 ± 0.4 9.8 ± 0.7 1.2 ± 0.1
R221C 14.8 ± 2.0 5.9 ± 0.4 3.2 ± 0.6
R354H 26.9 ± 2.5 5.1 ± 0.6 3.0 ± 0.4
W131G N.D. no binding N.D.
mutBACK N.D. 4.3 ± 0.3 N.D.
mutBTB N.D. 5.7 ± 0.4 N.D.
mutBTB-BACK N.D. 7.4 ± 0.4 N.D.
a

from CG-MAS; mean from at least three replicates ± S.D.

b

from fluorescence anisotropy assay; mean from at least three replicates ± S.D.

c

from fluorescence anisotropy assay; mean from at least three replicates ± S.D. N.D. = not determined